Bose Institute | Shubho Chaudhuri

Shubho Chaudhuri
Professor

Shubho Chaudhuri
Professor , Division of Plant Biology

PhD: University of Calcutta, 2001

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Previous appointments:

2002-2003: Post Doctoral Fellow, Department of Plant Pathology, University of Missouri, Columbia, USA

2003-2005: Post Doctoral fellow, Department of Horticulture, Washington State University, USA

2005-2009: Research coordinator, School of Molecular Biosciences, Washington State University, USA

Research interests:

Plants constantly modify their physiological processes in response to developmental and environmental cues. The reprogramming of cell differentiation in response to stimuli requires alteration of gene expressions, posttranscriptional and/or translational modifications of factors. Regulation of gene expression needs to occur in the context of chromatin. Change in chromatin landscape is therefore the rate-limiting step for such DNA-dependent processes. The central question therefore is to understand how gene expression is precisely regulated to give rise to the spatio-temporal response.

Contact:

Address:	Division of Plant Biology Centenary Campus Bose Institute P-1/12 C.I.T. Scheme VII-M Kolkata - 700054, India
E-Mail:	shubho[at]jcbose.ac.in
Phone:	+91-33-25693297

Research:

Investigating the role of ARID/HMG in modulation of chromatin structure in planta :

This project involves the study of a novel class of high mobility group (HMG) protein ARID-HMG known to modulate the chromatin structure during nuclear events. ARID-HMG proteins contain both the AT rich interaction domain (ARID) and the HMG-box domain in a single polypeptide. Of four the Arabidopsis ARID-HMG proteins, biochemical characterisations have been undertaken for AtHMGB11 (At1g55650) and AtHMGB15 (At1g04880), to understand the functionality of this protein and its individual domains. Our results indicate that ARID-HMG can bind different DNA topological forms, can bend DNA backbone and can also induce DNA supercoiling; hence facilitating nuclear processes. The present work involves a detailed biochemical and functional characterisation of AtHMGB11 and AtHMGB15.

The project also encompasses the study of key interacting partners of AtHMGB15. Protein-protein interaction study (Yeast two-hybrid assay) has revealed a Zinc finger C3HC4 RING/U-Box superfamily protein to interact with AtHMGB15 protein. Presently we are characterising the significance of this interaction.

Understanding the epigenetic regulation involved in transcription of abiotic stress induced genes in rice.

In this project we are investigating the epigenetic changes involved during the expression of stress responsive genes of rice. Abiotic stresses, such as, salinity and low temperature adversely affects the growth and development of plants. The study is also extended to a comparative epigenetic profiling in the context of rice plants for both sensitive and tolerant cultivars. Currently, a genome-wide study has been undertaken to identify changes in the epigenetic marks and co-relating it with the changes in the transcriptome when rice plants are subjected to different environmental stress.

Publications:

1. Deciphering the role of the AT-rich interaction domain and the HMG-box domain of ARID-HMG proteins of Arabidopsis thaliana. Adrita Roy, Arkajyoti Dutta, Dipan Roy, Payel Ganguly, Ritesh Ghosh, Rajiv K. Kar, Anirban Bhunia, Jayanta Mukhopadhyay, Shubho Chaudhuri. Plant Molecular Biology. (2016) 92:371–388. DOI 10.1007/s11103-016-0519-y

2. Differential acetylation of histone H3 at the regulatory region of OsDREB1b promoter facilitates chromatin remodelling and transcription activation during cold stress. Dipan Roy, Amit Paul, Adrita Roy, Ritesh Ghosh, Payel Ganguly and Shubho Chaudhuri. PloS One. 2014; Jun 18;9(6):e100343. DOI: 10.1371/journal.pone.0100343

3. Functional characterization of a serine-threonine protein kinase of Bambusa balcooa that implicates in cellulose overproduction and superior quality fiber formation. Jayadri S Ghosh, Shubho Chaudhuri, Nrisingha Dey and Amita Pal. BMC Plant Biology, (2013),13: 128 DOI:10.1186/1471-2229-13-128

4. Arabinosylated lipoarabinomannan skews Th2 phenotype towards Th1 during Leishmania infection by chromatin modification: involvement of MAPK signaling. Bhattacharya P, Gupta G, Majumder S, Adhikari A, Banerjee S, Halder K, Majumdar SB, Ghosh M, Chaudhuri S, Roy S, Majumdar S (2011) PLoS One. 2011;6(9):e24141 DOI: 10.1371/journal.pone.0024141

5. TLR signaling-mediated differential histone modification at IL-10 and IL-12 promoter region leads to functional impairments in tumor-associated macrophages. Banerjee S, Halder K, Bose A, Bhattacharya P, Gupta G, Karmahapatra S, Das S, Chaudhuri S, Bhattacharyya Majumdar S, Majumdar S (2011) Carcinogenesis. Dec;32(12):1789-97

6. Calcium/calmodulin -regulated Receptor-like Kinase is a positive regulator of Cold tolerance in Plants. Tianbao Yang, Shubho Chaudhuri, Lihua Yang, Liqun Du and B.W. Poovaiah (2010) J. Biol. Chem 285(10):7119-26.

7. Histone H3 Lys79 Methylation is Required for Efficient Nucleotide Excision Repair in a Silenced Locus of Saccharomyces cerevisiae. Shubho Chaudhuri, John J. Wyrick, and Michael J. Smerdon. (2009) Nucleic acids research 37(5): 1690-1700

8. Legume nodulation independent of rhizobia is induced by a modified calcium/calmodulin activated kinase lacking autoinhibition. Cynthia Gleason, Shubho Chaudhuri, Tianbao Yang, Alfonso Munoz-Gutierrez , B. W. Poovaiah, GIles Oldroyd. (2006) Nature 441: 1149–1152

9. Domain analysis of a groundnut calcium-dependent protein kinase: nuclear localization sequence in the junction domain is coupled with nonconsensus calcium binding domains. Raichaudhuri A, Bhattacharyya R, Chaudhuri S, Chakrabarti P, Dasgupta M. (2006) J. Biol. Chem., 281:10399-409

10. Calcium/calmodulin up-regulates a cytoplasmic receptor-like kinase in plants. Tianbao Yang, Shubho Chaudhuri, Lihua Yang, Yanping Chen, and B. W. Poovaiah. (2004) J. Biol. Chem., 279: 42552-42559

11. Identification of regulators of hrp/hop genes of Erwinia carotovora ssp. carotovora and characterization of HrpL_Ecc(sigma L_Ecc), an alternative sigma factor. Asita Chatterjee, Yaya cui, Shubho Chaudhuri and Arun Chatterjee (2002) Molecular Plant Pathology 3(5), 359-370

12. Exogenous auxin depletion renders Arachis hypogea suspension culture sensitive to water loss without affecting cell growth. Seal, A., Hazra A., Nag, R., Chaudhuri, S. and DasGupta, M (2001). Plant Cell Reports. 20(6): 567 – 573

13. Autophosphorylation Dependent Activation of a Calcium Dependent Protein Kinase from Arachis hypogea Plant. Subho Chaudhuri, Anindita Seal & Maitrayee DasGupta (1999). Plant Physiology 120: 859-866

BOOK CHAPTER:

CDPKS in plant signaling networks- Progress in research on Groundnut CDPK. In “Signal transduction in plants” (S.K. Sopory; R. Oelmuller and S. C. Maheshwari, Eds.). Maitrayee DasGupta and Subho Chaudhuri (2001). Kluwer academic Press, Page 145-156.

Recognition:

Teaching:

Present:

Image	Name	Designation	Department	Campus	Contact number	Email
	Jinia Chakrabartya	SRF	Division of Plant Biology	Centenary	25693297	jinia
	Pratiti Dasgupta	JRF	Division of Plant Biology	Centenary	25693297	pratitidgpt
	Rwitie Mallik	SRF	Division of Plant Biology	Centenary	25693297	rwitie
	Sanghamitra Adak	SRF	Division of Plant Biology	Centenary	25693254	sangha_s
	Sonal Sachdev	JRF	Division of Plant Biology	Centenary	25693297	sonalsachdev
	Trisha Bhattacharyya	JRF	Division of Plant Biology	Centenary	25693262