
Atin Kumar Mandal
Professor
Atin Kumar Mandal
Professor, Division of Molecular Medicine
PhD: Bose Institute, Jadavpur University, 2000
Previous appointments:
2015- Associate Professor, Division of Molecular Medicine, Bose Institute, Kolkata
2010-2015 Assistant Professor, Division of Molecular
Medicine, Bose Institute, Kolkata
2008-2010 Research Associate, Department of Biology, The City College of New York, New York
2005-2007 Post Doctoral Fellow, Department of Pharmacology and System Therapeutics, Mount Sinai School of Medicine, New York
2003-2005 Research Associate, Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Massachusetts
2000-2003 Post Doctoral Fellow, Department of Chemistry and
Biochemistry, Worcester Polytechnic Institute, Massachusetts
Research interests:
- Understanding the mechanism of protein kinase quality control in developement of pahtological conditions such as cancer, cardiac hypertrophy
- Crosstalk between molecular chaperones and ubiquitin ligases in quality control of misfolded aggregated prone proteins
- Develop inhibitors of molecular chaperones
Contact:
Address: |
Division of Molecular Medicine Centenary Campus Bose Institute P-1/12 C.I.T. Scheme VII-M Kolkata - 700054, India |
E-Mail: | mandalak[at]jcbose.ac.in |
Phone: | +91-33-25693237 |
Research:
Cellular protein quality control (PQC) maintains protein homeostasis at physiological or stress condition. Efficient clearance of misfolded proteins by refolding, degradation or sequestration is critical for cell survival. Molecular chaperones are integral part of PQC. Chaperones help in folding of several proteins linked with cell signaling and gene expression. Thus, chaperones become a potential target for various diseases including cancer, diabetes and even late-onset neurological diseases when efficiency of quality control decreases. However, quality control of such important chaperone clients such as protein kinases or misfolded proteins those are toxic for the cell is still elusive. Using both yeast and mammalian system I am trying to understand the mechanism of cellular protein quality control.
Misfolded kinase accumulates into distinct cellular quality control inclusions upon stress
Hsp90 mediated regulation of CRAF kinase
Publications:
1. Bhattacharyya D, Mohite
GM, Krishnamoorthy J, Gayen N, Mehra S, Navalkar
A, Kotler SA, Ratha BN, Ghosh A, Kumar R, Garai
K, Mandal AK, Maji SK, Bhunia A. Lipopolysaccharide from Gut Microbiota Modulates
α-Synuclein Aggregation and Alters Its Biological Function. ACS Chem Neurosci. 2019 Mar 20. doi:
10.1021/acschemneuro.8b00733
2. Mitra S, Ghosh B, Gayen N, Roy J & Mandal AK. Bipartite role of Hsp90 keeps CRAF poised for activation. Journal of Biological Chemistry, 291, 23545 - 23556 (2016)
3. Ratha BN, Ghosh A, Brender JR, Gayen
N, Ilyas H, Neeraja C, Das KP, Mandal AK & Bhunia A.
Inhibition of Insulin Amyloid Fibrillation by a Novel Amphipathic Heptapeptide:
Mechanistic Details Studied by Spectroscopy in Combination with Microscopy. Journal
of Biological Chemistry, 291,
24579 - 24593 (2016)
4. Roy J, Mitra S, Sengupta K & Mandal
AK. Hsp70 clears misfolded kinases that partitioned into distinct quality
control compartments. Molecular Biology of Cell, 26, 1583 - 1600
(2015)
5. Ghosh A, Ratha BN, Gayen N, Mroue KH,
Kar RK, Mandal AK*, Bhunia
A.* Biophysical Characterization of Essential Phosphorylation at the Flexible
C-Terminal Region of C-Raf with 14-3-3ζ Protein. PLoS One Aug 21;10(8) (2015) * Co-corresponding author
6. Roy
J & Mandal AK. Cellular
strategies to combat protein misfolding: Intricate role of Hsp70 in stress
management. Current Chemical
Biology, 8, 149 - 164 (2014)
7. Nillegoda NB, Theodoraki
MA, Mandal AK, Mayo KJ, Ren
HY, Sultana R, Wu K, Johnson J, Cyr DM, Caplan AJ. Ubr1 and Ubr2 function
in a quality control pathway for degradation of unfolded cytosolic proteins.
Molecular Biology of Cell,
21, 2102 - 2116 (2010).
8. Mandal AK*, Gibney PA*, Nillegoda NB, Theodoraki MA, Morano
KA & Caplan AJ. Hsp110 chaperones control client fate determination in the
Hsp70-Hsp90 chaperone system. Molecular
Biology of Cell 21, 1439 - 1448, 2010 *joint
first authors
9. Mandal AK, Nillegoda N, Chen J & Caplan AJ. Ydj1 protects
nascent protein kinases from degradation and controls the rate of their
maturation. Molecular and
Cellular Biology, 28 (13), 4434 - 4444
(2008)
10. Mandal AK, Lee P, Chen J, Nillegoda N, Heller H, DiStasio S, Oen
H, Victor J, Nair NM, Brodsky JL & Caplan AJ. Cdc37 has distinct roles in
protein kinase quality control that protects nascent chains from degradation
and promotes post-translational maturation. Journal of Cell Biology, 176, 319 - 328 (2007)
11. Caplan AJ, Mandal AK & Theodoraki MA. Molecular chaperones and
protein kinase quality control. Trends
in Cell Biology, 17(2), 87 - 92, (2007)
12. Robzyk K, Oen H, Buchanan G, Butler LM,
Tilley W, Mandal A K, Rosen
N, & Caplan AJ. Uncoupling of Hormone-dependence from chaperone-dependence
in the L701H mutation of the androgen receptor. Mol. Cell. Endocrinol., 268 (1-2):67 - 74, (2007)
13. Ying Y, Mandal AK, Bredeston LM, Gonzalez-Flecha FL & Argüello JM.
Activation of Archaeoglobus fulgidus Cu+-ATPase
CopA by cysteine. Biochim.
Biophys. Acta. 1768, 495 - 501 (2007)
14. Sazinsky MH, Mandal AK, Argüello JM, &
Rosenzweig AC. Structure of the ATP binding domain from the Archaeoglobus
fulgidus Cu+-ATPase. Journal of Biological Chemmistry, 281, 11161 - 11166 (2006)
15. Mandal AK, Yang Y, Kertesz TM & Argüello JM.
Identification of the transmembrane metal binding site in Cu+ transporting
P1B-type ATPases. Journal
of Biological Chemistry. 279, 54802 - 54807 (2004)
16. Mana-Capelli
S, Mandal AK, &
Argüello JM. Archaeoglobus fulgidus CopB is a thermophilic Cu2+-ATPase.
Functional role of its His-rich N-terminal metal binding domain. Journal of Biological Chemistry 278,
40534 - 40541 (2003)
17. Mandal AK & Argüello JM. Functional roles of
metal binding domains of the Archaeoglobus fulgidus Cu+-ATPase
CopA. Biochemistry, 42,
11040 - 11047 (2003)
18. Mandal
AK, Cheung W & Argüello, JM. Characterization of a thermophilic
P-type Ag+/Cu+ -ATPase from the extremophile Archaeoglobus
fulgidus. Journal of Biological
Chemistry, 277, 7201 - 7208, (2002)
19. Mikhaylova L, Mandal AK & Argüello JM. The
catalytic phosphorylation of the Na,K-ATPase drives the outward movement of the
cation-binding H5-H6 hairpin. Biochemistry, 41(25), 8195 - 8202, (2002)
20. Mandal AK, Roy K, Sil PC, Yadav SP & Sen PC.
Purification, characterization and partial amino acid sequencing of a 70 kD
inhibitor protein of Na+, K+-ATPase from goat testis
cytosol. Mol. Cell. Biochem. 223, 7 - 14
(2001)
21. Roy K, Mandal AK & Sen PC. A 75 kDa Na+,K+-ATPase
competitive inhibitor protein isolated from rat brain cytosol binds to a site
different from the ouabain-binding site. Eur. J. Biochem. 261, 84 - 88, 1999
22. Roy K, Mandal AK, Sikdar R, Majumder S, Ono Y & Sen PC.
Unsaturated fatty acid activated protein kinase (PKx) from goat testis cytosol. Biochim. Biophys. Acta. 1434,
161 - 169 (1999)
23. Sikdar R, Roy K, Mandal AK & Sen PC.
Phosphorylation and dephosphorylation of Mg2+-independent Ca2+-ATPase
from goat spermatozoa. J.
Bioscience. 24, 317 - 321 (1999)
Book Chapters
1. Mandal AK, Nair DM & Caplan AJ. Role of Cdc37 in protein kinase folding. In:“Protein Reviews” Vol. 7: Cell Stress Proteins (Edited by Stuart K. Calderwood) Springer. 326 - 337, 2007
2. Mandal
AK, Mikhaylova L & Argüello JM. S5-H5 helix in the Na,K-ATPase: A
structural link between phosphorylation and cation-binding sites. In: "The
Na,K-ATPase and Related Cation Pumps" (Jorgensen, P.L., Maunsbach, A.B.,
and S.J.D., Karlish, Eds.) Annals. New York Acad. Sc. 224-225 (2003)
3.
Argüello
JM, Mandal AK & Mana-Capelli S. Heavy metal transport CPx-ATPases from the
thermophile Archaeoglobus fulgidus.
In: "The Na,K-ATPase and Related Cation Pumps" (Jorgensen, P.L.,
Maunsbach, A.B., and S.J.D., Karlish, Eds.) Annals. New York Acad. Sc. 212-218 (2003)
Recognition:
Teaching:
Integrated Ph.D. course
Enzyme kinetics,
Cell stress response and Protein folding (MCB core)
PhD coursework
B.1. Enzymology
Students:
Image | Name | Designation | Department | Campus | Contact number | |
---|---|---|---|---|---|---|
![]() |
Dhiman Saha | Junior Research Fellow | Division of Molecular Medicine | Centenary | 25693338 | |
![]() |
Gourav Shome | Junior Research Fellow | Division of Molecular Medicine | Centenary | 25693231 | |
![]() |
Somesh Roy | Senior Research Fellow | Division of Molecular Medicine | Centenary | 25693338 | |
![]() |
Upama Chowdhury | Junior Research Fellow | Division of Molecular Medicine | Centenary | 0 |
Former:
Sahana Mitra, PhD, sahana.mitra84@gmail.com; Currently Post Doctoral Fellow at New York School of Medicine, New York, USA
Joydeep Roy, PhD. jroy4u@gmail.com; Currently Wenner-Gren Foundations Post Doctoral Fellow at Stockholm University, Stockholm
Debanjana Mitra, Project Assistant, shis.mitra@gmail.com; Currently at Biocon Biopharmaceutical Company, Bangalore
Group News:
- Sept 08, 2018- Nilanjan Gayen gave a talk in one day symposium at Amity University organised by SBC(I), Kolkata Chapter
- Baijayanti Ghosh received EMBO travel award to attend EMBO Workshop on ‘Protein quality control: From mechanisms to disease’; 28 April -03 May 2019, Costa de la Calma (Mallorca), Spain
- Congratulation Baijayanti for winning FEBS LETTER poster prize at EMBO workshop